Unraveling Epistasis With Triple Testcross Progenies of Near-Isogenic Lines

Unraveling epistasis with triple testcross progenies of near-isogenic lines.

Libraries of near-isogenic lines (NILs) are a powerful plant genetic resource to map quantitative trait loci (QTL). Nevertheless, QTL mapping with NILs is mostly restricted to genetic main effects. Here we propose a two-step procedure to map additive-by-additive digenic epistasis with NILs. In the first step, a generation means analysis of parents, their F(1) hybrid, and one-segment NILs and th...

Unravelling epistasis with triple testcross progenies of near 1 isogenic lines 2 3 4 5

Genetic basis of heterosis for growth-related traits in Arabidopsis investigated by testcross progenies of near-isogenic lines reveals a significant role of epistasis.

Epistasis seems to play a significant role in the manifestation of heterosis. However, the power of detecting epistatic interactions among quantitative trait loci (QTL) in segregating populations is low. We studied heterosis in Arabidopsis thaliana hybrid C24 x Col-0 by testing near-isogenic lines (NILs) and their triple testcross (TTC) progenies. Our objectives were to (i) provide the theoreti...

Analysis of a triple testcross design with recombinant inbred lines reveals a significant role of epistasis in heterosis for biomass-related traits in Arabidopsis.

Primary causes of heterosis are still unknown. Our goal was to investigate the extent and underlying genetic causes of heterosis for five biomass-related traits in Arabidopsis thaliana. We (i) investigated the relative contribution of dominance and epistatic effects to heterosis in the hybrid C24 x Col-0 by generation means analysis and estimates of variance components based on a triple testcro...

Protein and Quality Characterization of Pegaso Wheat Near-Isogenic Lines

Susceptibility of Lasioderma serricorne (F.) (Coleoptera: Anobiidae) Life Stages to Elevated Temperatures Used During Structural Heat Treatments Authors: C. Yu, B. Subramanyam, P.W. Flinn, J.A. Gwirtz Submitted to: Journal of Economic Entomology Methyl bromide is still widely used in food processing and storage facilities for the suppression of storedproduct insect pests, but its use is schedul...